The interaction between human and bovine serum albumin and zinc studied by a competitive spectrophotometry.

@article{Ohyoshi1999TheIB,
  title={The interaction between human and bovine serum albumin and zinc studied by a competitive spectrophotometry.},
  author={E Ohyoshi and Yumi Hamada and Kou Nakata and Satoshi Kohata},
  journal={Journal of inorganic biochemistry},
  year={1999},
  volume={75 3},
  pages={213-8}
}
The binding of zinc to human serum albumin (HSA) and bovine serum albumin (BSA) has been studied by a competitive spectrophotometry. The indictor ligand, 5-Br-PAPS, as well as its metal complexes, ML and ML2, having intense colors with sufficient separate absorption peaks permits the analysis of the interaction between zinc and HSA or BSA. The results show that zinc binds to both albumins in the molar ratio of 1:1 at pH 6-8. The apparent association constants of Zn-HSA and Zn-BSA decrease with… CONTINUE READING