The interaction between different domains of staphylococcal protein A and human polyclonal IgG, IgA, IgM and F(ab')2: separation of affinity from specificity.

@article{Ljungberg1993TheIB,
  title={The interaction between different domains of staphylococcal protein A and human polyclonal IgG, IgA, IgM and F(ab')2: separation of affinity from specificity.},
  author={U K Ljungberg and Birger O Jansson and U Niss and Robert Nilsson and Bengt E B Sandberg and B. W. Nilsson},
  journal={Molecular immunology},
  year={1993},
  volume={30 14},
  pages={1279-85}
}
Binding properties of staphylococcal protein A (SpA) to different human immunoglobulins have been investigated. In this analysis, intact SpA as well as SpA-derived fragments containing one to five IgG-binding domains of different compositions, were used. The affinity binding constants of the different proteins to human polyclonal IgG, IgA, IgM and F(ab')2-fragments as well as their binding capacity to the immunoglobulin molecules were determined. The results show that although all the proteins… CONTINUE READING