The integral membrane protein p16.7 organizes in vivo phi29 DNA replication through interaction with both the terminal protein and ssDNA.

@article{SernaRico2003TheIM,
  title={The integral membrane protein p16.7 organizes in vivo phi29 DNA replication through interaction with both the terminal protein and ssDNA.},
  author={Alejandro Serna-Rico and Daniel Mu{\~n}oz-Esp{\'i}n and Laurentino Villar and M. Salas and Wilfried J J Meijer},
  journal={The EMBO journal},
  year={2003},
  volume={22 9},
  pages={
          2297-306
        }
}
Remarkably little is known about the in vivo organization of membrane-associated prokaryotic DNA replication or the proteins involved. We have studied this fundamental process using the Bacillus subtilis phage phi29 as a model system. Previously, we demonstrated that the phi29-encoded dimeric integral membrane protein p16.7 binds to ssDNA and is involved in the organization of membrane-associated phi29 DNA replication. Here we demonstrate that p16.7 forms multimers, both in vitro and in vivo… CONTINUE READING
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