The insulinomimetic agents H2O2 and vanadate stimulate protein tyrosine phosphorylation in intact cells.

@article{Heffetz1990TheIA,
  title={The insulinomimetic agents H2O2 and vanadate stimulate protein tyrosine phosphorylation in intact cells.},
  author={D Heffetz and I Bushkin and Rinat Dror and Yehiel Zick},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 5},
  pages={2896-902}
}
H2O2 and vanadate are known insulinomimetic agents. Together they induce insulin's bioeffects with a potency which exceeds that seen with insulin, vanadate, or H2O2 alone. Employing Western blotting with anti-P-Tyr antibodies, we have identified in Fao cells at least four proteins (pp180, 150, 114, and 100) whose P-Tyr content is rapidly increased upon treatment of the cells with 3 mM H2O2. Tyrosine phosphorylation of these and additional proteins was markedly potentiated (6-10-fold) when 100… CONTINUE READING