The inside scoop—evaluating gene delivery methods

  title={The inside scoop—evaluating gene delivery methods},
  author={Laura P. Bonetta},
  journal={Nature Methods},
  • L. Bonetta
  • Published 1 November 2005
  • Biology
  • Nature Methods
Techniques for delivering nucleic acids into mammalian cells have been around for decades. But tools and reagents continue to improve and target a broader range of cells and applications. Laura Bonetta reports. 
Engineered nonviral nanocarriers for intracellular gene delivery applications.
The different material platforms for gene delivery are comparatively addressed, and the mechanisms of interaction with biological systems are discussed carefully.
A Study of Cultured Cells on a Nanowire-based Reverse Transfection Device
This work builds on previous research at NTNU into the use of vertically aligned copper oxide nanowire arrays as a versatile system to deliver biomolecules into cultured adherent mammalian cells. T
Chapter 11 – Gene Delivery Strategies
The purpose of this chapter is to survey the common methods of delivering recombinant DNA into cells in each of the environments a neuroscientist might want to deliver a DNA sequence into a population of cells in culture, in a brain slice, or in the brain of a living animal.
Single cell transfection with single molecule resolution using a synthetic nanopore.
We report the development of a single cell gene delivery system based on electroporation using a synthetic nanopore, that is not only highly specific and very efficient but also transfects with
Extended and stable gene expression via nucleofection of MIDGE construct into adult human marrow mesenchymal stromal cells
Nucleofection is an efficient non-viral transfection approach for h MSC, which when used in conjunction with a MIDGE construct, could result in extended and stable transgene expression in hMSC.
Gene Delivery Strategies
This chapter is to survey the common methods of delivering recombinant DNA into cells in each of these environments, including physical, chemical, and viral methods.
Delivery of Short Interfering Ribonucleic Acid-Complexed Magnetic Nanoparticles in an Oscillating Field Occurs via Caveolae-Mediated Endocytosis
A novel gene delivery technique is developed, which uses magnetic nanoparticles moving under the influence of an oscillating magnetic array to introduce short interfering RNA (siRNA) against green fluorescent protein (GFP) or actin into stably-transfected GFP-HeLa cells or wild-type HeLa and rat aortic smooth muscle cells.
Magnetic nanoparticle formulations for DNA and siRNA delivery
Newly synthesized magnetic nanomaterials possess high DNA binding capacity either itself or in the presence of a positively charged lipid-based Metafectene™ reagent or branched polyethylene imine 25
Non-Viral in Vitro Gene Delivery: It is Now Time to Set the Bar!
This review provides a catalog of conditions optimized for the gold standard and internal reference, 25 kDa polyethyleneimine, that can be profitably replicated across studies for the sake of comparison and pave the way for the implementation of standardized protocols in order to make the evaluation of the effectiveness of transfectants as unbiased as possible.
Self-assembled magnetic theranostic nanoparticles for highly sensitive MRI of minicircle DNA delivery.
An MRI visible gene delivery system developed with a core of SPIO nanocrystals and a shell of biodegradable stearic acid-modified low molecular weight polyethyleneimine (Stearic-LWPEI) via self-assembly shows synergistic advantages in the effective transfection of mcDNA and non-invasive MRI of gene delivery.


Photochemical internalization: a novel technology for delivery of macromolecules into cytosol.
Results presented here show that PCI can induce efficient light-directed delivery of macromolecules into the cytosol, indicating that PCI may have a variety of useful applications for site-specific drug delivery, e.g., in gene therapy, vaccination, and cancer treatment.
Transformation of rat cells by DNA of human adenovirus 5.
Primary rat embryo and baby rat kidney cells have been transformed by human adenovirus 5 DNA and the efficiency was approximately 1 transformed focus/μg DNA.
Localized transfection on arrays of magnetic beads coated with PCR products
A simple system that allows any molecular biology laboratory to carry out multiple, parallel cell transfections on microscope coverslip arrays by using magnetically defined positions and PCR product–coated paramagnetic beads to identify previously unknown cellular localization motifs is described.
Microarrays of cells expressing defined cDNAs
A microarray-driven gene expression system for the functional analysis of many gene products in parallel is developed and identified proteins involved in tyrosine kinase signalling, apoptosis and cell adhesion, and with distinct subcellular distributions.
Engineering Gene Networks to Emulate Drosophila Embryonic Pattern Formation
Artificial transcription-translation networks are engineered that generate simple patterns, crudely analogous to the Drosophila gap gene system, and it is found that for pattern formation to occur under the conditions of the in vitro reaction-diffusion system, the activator molecules must propagate faster than the inhibitors.