The insecticide spinosad induces DNA damage and apoptosis in HEK293 and HepG2 cells.

Abstract

Spinosad, a pesticide acting on the central nervous system of insects, is classified as a pesticide with reduced risk. However, spinosad-induced toxicological effects on non-target organisms must not be ignored. This study aimed to evaluate the cytotoxicity and potential genotoxicity of spinosad in HEK293 and HepG2 cell lines. The results showed that spinosad caused a concentration- and time-dependent decrease in cell viability of HEK293 and HepG2 cells. Spinosad-induced p53 accumulation thereby upregulates the expression of Bax and downregulates the expression of Bcl-2. Further studies confirmed that spinosad induced apoptosis in HEK293 and HepG2 cells, accompanied by a dissipation of the mitochondrial membrane potential and an increase in caspase-3 activity. The alkaline comet assay and γ-H2AX foci staining revealed that spinosad induced significant concentration-dependent increases of DNA strand breaks in HEK293 and HepG2 cells. Our results indicate that spinosad effectively induced DNA damage and apoptosis in HEK293 and HepG2 cells.

DOI: 10.1016/j.mrgentox.2016.11.001

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Cite this paper

@article{Yang2016TheIS, title={The insecticide spinosad induces DNA damage and apoptosis in HEK293 and HepG2 cells.}, author={Mingjun Yang and Guanggang Xiang and Diqiu Li and Yang Zhang and Wenping Xu and Liming Tao}, journal={Mutation research}, year={2016}, volume={812}, pages={12-19} }