The inhibition of mitochondrial F1-ATPase by 1,5-difluoro-2,4-dinitrobenzene.

  title={The inhibition of mitochondrial F1-ATPase by 1,5-difluoro-2,4-dinitrobenzene.},
  author={A. A. Akhrem and Mikhail A Kisel and Igor A. Kozlov and I S Tsybovsky and Evgeny N. Vulfson},
  journal={FEBS letters},
  volume={187 2},
1,5-Difluoro-2,4-dinitrobenzene completely inhibits F1-ATPase when it is used at micromolar concentrations and the inhibitor/enzyme molar ratio is equal to 3. The inhibition can be reversed by dithiothreitol treatment. 7-Chloro-4-nitrobenzofurazan treatment of F1-ATPase does not prevent the reaction of the enzyme with 1,5-difluoro-2,4-dinitrobenzene. The 1,5-difluoro-2,4-dinitrobenzene-induced inhibition is thought to be a result of the modification of a tyrosine residue with pK 9.1.