The inheritance of chemical phenotype in Cannabis sativa L. (II): Cannabigerol predominant plants

  title={The inheritance of chemical phenotype in Cannabis sativa L. (II): Cannabigerol predominant plants},
  author={Etienne de Meijer and K. M. Hammond},
This paper aims to clarify the genetic mechanism that is responsible for the accumulation of cannabigerol (CBG) in certain phenotypes of Cannabis sativa L. CBG is the direct precursor of the cannabinoids CBD, THC and CBC. [] Key Method Inbred offspring derived from one such individual were crossed with true breeding THC predominant- and CBD predominant plants, respectively. The segregations in the cross progenies indicate that CBG accumulation is due to the homozygous presence of a minimally functional…

The inheritance of chemical phenotype in Cannabis sativa L. (III): variation in cannabichromene proportion

The mechanism that controls the proportion of cannabichromene (CBC), a potential pharmaceutical, in the cannabinoid fraction of Cannabis sativa L. is explored and the ability of PJC plants to enable the production of pharmaceutical raw material with high CBC purity is demonstrated.

The inheritance of chemical phenotype in Cannabissativa L. (IV): cannabinoid-free plants

Backcrossing produced cannabinoid-free homologues of pharmaceutical production clones with potential applications in pharmacological research and suggests that locus O governs a reaction in the pathway towards the phenolic cannabinoid precursors.

SNP in Potentially Defunct Tetrahydrocannabinolic Acid Synthase Is a Marker for Cannabigerolic Acid Dominance in Cannabis sativa L.

It is confirmed that CBGA dominance is inherited as a single recessive gene, potentially governed by a non-functioning allelic variant of the THCA synthase, and a single nucleotide polymorphism (SNP) that may render the synthase unable to convert CBGA to THCA leading to the accumulation of CBGA.

Cannabinoid Inheritance Relies on Complex Genetic Architecture.

Cannabinoid inheritance is more complex than previously appreciated; among other genetic effects, cytogenetic and maternal contributions may be undervalued influences on cannabinoid ratios and concentrations.

The inheritance of chemical phenotype in Cannabis sativa L. (V): regulation of the propyl-/pentyl cannabinoid ratio, completion of a genetic model

Inbred lines derived from multi-cross hybrid combinations reached unprecedented PC3 levels of up to 96 % which supports the hypothesis of the regulation of C3/C5 ratios, and a model of a multiple locus A1–A2–…An is proposed, with the pentyl- and propyl cannabinoid pathway being enhanced by alleles Ape1−n and Apr1–n, respectively.

Chemical and Morphological Phenotypes in Breeding of Cannabis sativa L.

This chapter details five characters that contribute to phenotypic diversity in Cannabis and dissects the monogenic inheritance model (two alleles at a single gene locus).

Time course of cannabinoid accumulation and chemotype development during the growth of Cannabis sativa L

It was confirmed that callus cultures of Cannabis were not able to produce detectable amounts of any cannabinoids, irrespective of the type and amount of cannabinoids in the mother plants.

Genetics and Marker-assisted Selection of the Chemotype in Cannabis sativa L.

The effectiveness of two different types of markers associated to the locus determining the chemotype in Cannabis was evaluated and discussed, as possible tools in marker-assisted selection in hemp, but also for possible applications in the forensic and pharmaceutical fields.

The Cream of the Crop: Biology, Breeding, and Applications of Cannabis Sativa

The biology of C. sativa is reviewed, including recent insights from taxonomy, morphology and genomics, with an emphasis on the genetics of cannabinoid synthesis, as well as the tremendous medicinal, engineering, and environmental opportunities that Cannabis bears.



A chemotaxonomic analysis of cannabinoid variation in Cannabis (Cannabaceae).

A two-species concept of Cannabis is supported by the determined frequencies of B(D) and B(T) in sample populations of 157 Cannabis accessions determined from CBD and THC banding patterns, visualized by starch gel electrophoresis.

Identification of a new chemotype in Cannabis sativa: cannabigerol-dominant plants, biogenetic and agronomic prospects.

The present paper provides the description of a highly interesting, new, “fourth“ chemotype, whose very low content in Δ9-tetrahydrocannabinol (Δ9-THC) (0.001%) in contrast with the high amounts which occur in cannabigerol (CBG), its major cannabinoīd component.

Purification and Characterization of Cannabidiolic-acid Synthase from Cannabis sativa L.

It is concluded that CBDA is predominantly biosynthesized from cannabigerolic acid rather than cannabinerolic acid, although the kcat for the former is lower than that for the latter, and that the enzymatic cyclization does not proceed via oxygenated intermediates.

Again on the Nature of Inheritance of Chemotype

In Vol. 7, no. 1, 2002 of JIH, Karl Hillig reviewed a paper by Sytnik and Stelmah published in JIHA (Vol. 6, no. 1, 1999). Hillig criticized the conclusions drawn by the authors from their

Purification and characterization of cannabichromenic acid synthase from Cannabis sativa.

Enzymological Evidence for Cannabichromenic Acid Biosynthesis

An enzyme involved in the biosynthesis of cannabichromenic acid was identified in young leaves of Cannabis sativa, similar to those of Δ1-tetrahydrocannabinolic acid and cannabidiolic acid.

Cannabinoids with a Propyl Side Chain in Cannabis: Occurrence and Chromatographic Behavior

In this chapter, detection and identification of the propyl and pentyl cannabinoids in gas chromatography and thin-layer chromatography is discussed.

Isolation, Structure, and Partial Synthesis of an Active Constituent of Hashish

strongly solvated than triple ions,lb-5 the difference being greater for cations of smaller radii, leading to a trend to smaller K with decreasing cationic radii. Both effects, in opposite