The influence of temperature and length of time of storage of frog mucus samples.

@article{Gastaldi2000TheIO,
  title={The influence of temperature and length of time of storage of frog mucus samples.},
  author={Ada Clarice Gastaldi and Jos{\'e} Roberto de Brito Jardim and Malcolm King},
  journal={Biorheology},
  year={2000},
  volume={37 3},
  pages={203-11}
}
Frozen, stored mucus has been extensively used for transport studies but there is no clear evidence of the influence that the temperature and length of time of storage may have on the results. We stored frog mucus samples at -20 and -80 degrees C and analysed them on days zero, 2, 10, 30 and 90. At each temperature, a sample was thawed, studied and refrozen on each of the study days, at the same time that one sample was thawed only on the study day. Displacement in a simulated cough machine and… CONTINUE READING

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