The influence of movement on the localization precision of sub-resolution particles in fluorescence microscopy.

@article{Deschout2012TheIO,
  title={The influence of movement on the localization precision of sub-resolution particles in fluorescence microscopy.},
  author={Hendrik Deschout and Kristiaan Neyts and Kevin Braeckmans},
  journal={Journal of biophotonics},
  year={2012},
  volume={5 1},
  pages={97-109}
}
Obtaining sub-resolution particle positions in fluorescence microscopy images is essential for single particle tracking and high-resolution localization microscopy. While the localization precision of stationary single molecules or particles is well understood, the influence of particle motion during image acquisition has been largely neglected. Here, we address this issue and provide a theoretical description on how particle motion influences the centroid localization precision, both in case… CONTINUE READING

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