The influence of different erythrocyte lysing procedures on flow cytometric determination of CD34+ cells in umbilical cord blood transplants.

@article{Cassens1998TheIO,
  title={The influence of different erythrocyte lysing procedures on flow cytometric determination of CD34+ cells in umbilical cord blood transplants.},
  author={Uwe Cassens and Kai Gutensohn and Henk S. P. Garritsen and Reinhard Kelsch and Peter Kuehnl and Walter Sibrowski},
  journal={Transfusion medicine},
  year={1998},
  volume={8 2},
  pages={
          111-8
        }
}
Since the correct determination of CD34+ cells is of great clinical importance for successful transplantation with haematopoietic progenitor cells (HPCs) from cord blood, we investigated the influence of different erythrocyte lysing techniques on the quantification of CD34+ cells in umbilical cord blood. Flow cytometric determinations of CD34+ cells were performed from 20 cord blood samples, using three different erythrocyte lysing procedures and two monoclonal CD34 antibodies (n = 360). Flow… CONTINUE READING
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