The influence of 2,3-butanedione 2-monoxime (BDM) on the interaction between actin and myosin in solution and in skinned muscle fibres

@article{Mckillop1994TheIO,
  title={The influence of 2,3-butanedione 2-monoxime (BDM) on the interaction between actin and myosin in solution and in skinned muscle fibres},
  author={D. F. A. Mckillop and N. S. Fortune and Kemachandra Ranatunga and Michael A. Geeves},
  journal={Journal of Muscle Research & Cell Motility},
  year={1994},
  volume={15},
  pages={309-318}
}
2,3-butanedione 2-monoxime (BDM) inhibits muscle contraction and actomyosin ATPase both in fibres and in solution. It is potentially useful as a tool for exploring weak interactions between actin and myosin. We have examined the effect of BDM on several key steps of the myosin subfragment-1 and actomyosin subfragment-1 ATPase in solution. These studies show that BDM shifts the equilibrium between two actomyosin states towards a more weakly bound form when the acto.myosin complex has ADP alone… CONTINUE READING

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