The infectious BAC genomic DNA expression library: a high capacity vector system for functional genomics


Gene dosage plays a critical role in a range of cellular phenotypes, yet most cellular expression systems use heterologous cDNA-based vectors which express proteins well above physiological levels. In contrast, genomic DNA expression vectors generate physiologically-relevant levels of gene expression by carrying the whole genomic DNA locus of a gene… (More)
DOI: 10.1038/srep28644