The induction of pyruvate kinase synthesis by heat shock in Xenopus laevis embryos.

@article{Marsden1993TheIO,
  title={The induction of pyruvate kinase synthesis by heat shock in Xenopus laevis embryos.},
  author={Marion Marsden and R W Nickells and M. S. Chetan Kapoor and Leon W. Browder},
  journal={Developmental genetics},
  year={1993},
  volume={14 1},
  pages={51-7}
}
Heat-shocked Xenopus embryos have an unusually complex heat shock response. The dominant heat shock protein (Hsp) has a relative molecular mass (M(r)) of 62,000 D (Hsp62). Affinity-purified IgGs against the glycolytic enzyme pyruvate kinase (PK; EC 2.7.1.40) specifically immunoprecipitated Hsp62 from extracts of embryos that had been heat-shocked at 37 degrees C for 30 min. Thus, Hsp62 and pyruvate kinase are immunologically cross-reacting. Electrophoretic separation of PK isoforms suggests… CONTINUE READING

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