The inducible and cytochrome P450-containing dehydroepiandrosterone 7α-hydroxylating enzyme system of Fusarium moniliforme

@article{Cotillon1997TheIA,
  title={The inducible and cytochrome P450-containing dehydroepiandrosterone 7$\alpha$-hydroxylating enzyme system of Fusarium moniliforme
},
  author={A. C. Cotillon and Jaleh Doostzadeh and Robert Morfin},
  journal={The Journal of Steroid Biochemistry and Molecular Biology},
  year={1997},
  volume={62},
  pages={467-475}
}

Transformation of 3-hydroxy-steroids by Fusarium moniliforme 7α-hydroxylase

  • A. CotillonR. Morfin
  • Chemistry, Biology
    The Journal of Steroid Biochemistry and Molecular Biology
  • 1999

Hydroxylation of DHEA, androstenediol and epiandrosterone by Mortierella isabellina AM212. Evidence indicating that both constitutive and inducible hydroxylases catalyze 7α- as well as 7β-hydroxylations of 5-ene substrates.

Ketoconazole and cycloheximide inhibition studies suggest the presence of constitutive and substrate-induced hydroxylases in M. isabellina on the basis of time course analysis of theHydroxylation of DHEA and androstenediol.

The human cytochrome P4507B1: catalytic activity studies

Screening of mycelial fungi for 7α- and 7β-hydroxylase activity towards dehydroepiandrosterone

In total, 481 fungal strains were screened for the ability to carry out 7(α/β)-hydroxylation of dehydroepiandrosterone (DHEA, 3β-hydroxy-5-androsten-17-one), and the majority of strains were able to introduce a hydroxyl group to position 7α.

Dehydroepiandrosterone Metabolites and their Interactions in Humans

The cytochrome P4507B1 responsible for 7alpha-hydroxy-DHEA production and 11beta-HSD1 are key enzymes for the modulation of glucocorticoid action in humans.

Dihydroxylation of dehydroepiandrosterone in positions 7α and 15α by mycelial fungi

Application of the revealed active strains of microorganisms opens prospects for the efficient production of key intermediates of synthesis of modern medical preparations.

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The quantitatively important metabolism to 7 alpha-OH compounds may contribute to the control of neurosteroid activity in brain, and derivatives of DHEA and PREG are known to be neuroactive.

Pregnenolone-7 beta-hydroxylating activities of yeast-expressed mouse cytochrome P450-1A1 and mouse-tissue microsomes.

Findings indicate that, in addition to unidentified P450, P450 1A1 is involved in 7 beta-hydroxylation of pregnenolone and may contribute in part to the production of the 7-Hydroxylated steroids necessary for activation of immune defenses.

Pregnenolone-7β-hydroxylating activity of human cytochrome P450-1A1