The incorporation of solubilized choline-transport activity into liposomes.

@article{King1982TheIO,
  title={The incorporation of solubilized choline-transport activity into liposomes.},
  author={Ron G. King and Robert Marchbanks},
  journal={The Biochemical journal},
  year={1982},
  volume={204 2},
  pages={565-76}
}
The choline-transport system has been solubilized from synaptic plasma membrane by using either sodium cholate or Triton X-100, and re-incorporated into unilamellar liposomes by using the technique of cholate dialysis. The criteria of choline-transport activity were saturability by excess choline, inhibition by hemicholinium-3, and trans-activation (i.e. stimulation of the uptake of [3H]choline into liposomes by preloading them with non-radioactive choline). Liposomes prepared from detergent… CONTINUE READING