The in vitro uptake and metabolism of peptides and amino acids by five species of rumen bacteria.

@article{Ling1995TheIV,
  title={The in vitro uptake and metabolism of peptides and amino acids by five species of rumen bacteria.},
  author={J. R. Ling and I. Armstead},
  journal={The Journal of applied bacteriology},
  year={1995},
  volume={78 2},
  pages={
          116-24
        }
}
Streptococcus bovis JB1, Prevotella ruminicola B(1)4, Selenomonas ruminantium Z108, Fibrobacter succinogenes S85 and Anaerovibrio lipolytica 5S were incubated with either 14C-peptides (mol. wt, 200-1000) or 14C-amino acids to compare their rates of uptake and metabolism. In experiment 1, the bacteria were grown and incubated in a complex medium, but no uptake of 14C-labelled substrates occurred. When casein digest was omitted, uptake rates of 14C-peptides were different (P < 0.01) with each… Expand
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References

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Variations in the uptake and metabolism of peptides and amino acids by mixed ruminal bacteria in vitro
TLDR
Mixed ruminal bacteria, isolated from sheep (Q and W) fed a concentrate and hay diet, were anaerobically incubated with either 14C-peptides or14C-amino acids, and radioactivity was contained only in tyrosine, phenylalanine, and mostly proline for incubation but in up to 10 amino acids when 15C- peptides were the substrates. Expand
Fermentation of peptides and amino acids by a monensin-sensitive ruminal Peptostreptococcus
A monensin-sensitive ruminal peptostreptococcus was able to grow rapidly (growth rate of 0.5/h) on an enzymatic hydrolysate of casein, but less than 23% of the amino acid nitrogen was ever utilized.Expand
Fermentation of Peptides by Bacteroides ruminicola B14
  • James B. Russell
  • Medicine, Biology
  • Applied and environmental microbiology
  • 1983
TLDR
In glucose-limited chemostats, ammonia production from peptides was inversely proportional to the dilution rate, and 87% of the variation in ammonia production could be explained by retention time in the culture vessel. Expand
More monensin-sensitive, ammonia-producing bacteria from the rumen
TLDR
The irregularly shaped cells and short rods were present at less than 10(9)/ml in vivo, but they ahd very high specific activities of ammonia production and could play an important role in ruminal amino acid fermentation. Expand
PEPTIDES AND OTHER NITROGEN SOURCES FOR GROWTH OF BACTEROIDES RUMINICOLA.
TLDR
Representative strains of Bacteroides ruminicola were found to utilize peptide nitrogen or ammonia nitrogen, but not to utilize significant amounts of free amino acid nitrogen or the nitrogen from a variety of other low molecular weight compounds for growth. Expand
Effect of hydrophobicity of utilization of peptides by ruminal bacteria in vitro
TLDR
Pure cultures of ruminal bacteria had a similar preference for hydrophilic peptides and likewise utilized peptides at a faster rate than free amino acids, suggesting hydrophobicity is likely to influence the composition of amino acids passing unfermented to the lower gut of ruminant animals. Expand
Oligopeptide Uptake by Bacteroides ruminicola
TLDR
It is concluded that B. ruminicola possesses a general system for the uptake of peptides, that peptides are rapidly hydrolyzed during or after uptake, and that oligopeptides function only to supply amino acids in a form available to the organism. Expand
Amino acid uptake systems in Bacteroides ruminicola.
  • R. Stevenson
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TLDR
The failure of free amino acids to act as sole nitrogen sources for growth of B. ruminicola is not due to the absence of transport systems for these compounds, as it is shown that multiple uptake systems were involved. Expand
Proteolytic activity of the ruminal bacterium Butyrivibrio fibrisolvens.
TLDR
The proteolytic activity of Butyrivibrio fibrisolvens, a ubiquitously distributed bacterial species in the gastrointestinal tracts of ruminants and other mammals, was characterized and was positively correlated with the growth rate; however, when the grow rate varied with the use of different nitrogen sources, a similar correlation was not found. Expand
Selective isolation of bacteria with dipeptidyl aminopeptidase type I activity from the sheep rumen.
TLDR
It was concluded that B. ruminicola forms the most important group of bacteria in the rumen to possess a dipeptidyl aminopeptidase type I activity. Expand
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