The in vitro hydrolysis of phytosterol conjugates in food matrices by mammalian digestive enzymes

@article{Moreau2004TheIV,
  title={The in vitro hydrolysis of phytosterol conjugates in food matrices by mammalian digestive enzymes},
  author={Robert A. Moreau and Kevin B. Hicks},
  journal={Lipids},
  year={2004},
  volume={39},
  pages={769-776}
}
All fruits, vegetables, and grains contain phytosterols. Numerous clinical studies have documented that phytosterols lower LDL-cholesterol levels and thereby reduce the risk of cardiovascular disease. Most experts believe that the cholesterol-lowering mechanism of phytosterols requires that they be in their “free” form. In addition to their occurrence in the free form, phytosterols also occur as four common phytosterol conjugates: (i) fatty acyl esters, (ii) hydroxycinnamate esters, (iii… 

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References

SHOWING 1-10 OF 26 REFERENCES

Improved method for the synthesis of trans-feruloyl-beta-sitostanol.

TLDR
The process was successfully applied to synthesize stanol trans-feruloyl esters from "Vegetable Stanols", a mixture of approximately 70:30 beta-sitostanol and beta-campestanol, in comparable purity and yield.

Evaluation of a commercial enzyme-based serum cholesterol test kit for analysis of phytosterol and phytostanol products.

TLDR
A popular commercial spectrophotometric serum cholesterol test kit was evaluated and results indicate that the method could be modified to analyze phytosterols and phytostanols by increasing the incubation time.

Metabolism of sitosteryl β-D-glucoside and its nutritional effects in rats

TLDR
Analyses of steryl lipids of the various organs and tissues confirmed the findings obtained with the radioactive substrate: neither sitosteryl β-D-glucoside nor sitosterol orSitosteryl esters derived therefrom had been transported in appreciable amounts to organs and tissue outside the alimentary canal during the feeding period.

Metabolic effects of plant sterols and stanols (Review).

Xylanase treatment of plant cells induces glycosylation and fatty acylation of phytosterols

TLDR
It is demonstrated that xylanase from Trichoderma viride induces a dramatic shift in the level of acylated sterol glycosides, indicating that endoxylanase was probably the active component in the cellulase enzyme preparations used in the previous study.

Absorption and metabolism of γ-oryzanol in rats

TLDR
It is considered that γ-oryzanol administered orally is absorbed mainly into the blood via the portal vein system, not into the lymph via the thoracic duct.

Quantitation of steryl ferulate andp-coumarate esters from corn and rice

TLDR
The data presented provide a direct comparison of steryl ferulate andp-coumarate levels in the two cereals, and will aid in selecting the most suitable sources for the isolation of these compounds from corn products.

Serum, biliary, and fecal cholesterol and plant sterols in colectomized patients before and during consumption of stanol ester margarine.

TLDR
Small amounts of plant stanols are recovered in serum and bile during consumption of stanol esters but effectively are secreted through bile, thereby balancing the intake-induced increase in their absorption.

Effect of esterified 4-desmethylsterols and -stanols or 4,4′-dimethylsterols on cholesterol and bile acid metabolism in hamsters

TLDR
Esterified sterols and stanols were equally effective in lowering plasma TC and LDL cholesterol, while dimethylsterol esters caused a weaker cholesterol-lowering effect.