The in vitro culture of primitive-streak-stage mouse embryos.

  title={The in vitro culture of primitive-streak-stage mouse embryos.},
  author={Patrick P. L. Tam and Mikel H. Snow},
  journal={Journal of embryology and experimental morphology},
The in vitro growth and morphogenesis of mouse embryos from early primitive-streak stage to early-somite stage is described. The embryo culture method employs a static culture system, a conventional chemically defined medium (Dulbecco's modified Eagle's medium), supplemented with additional glucose, glutamine and suitably prepared serum. The method of serum preparation is important for successful culture. Both mouse serum and rat serum support good development of primitive-streak-stage mouse… CONTINUE READING

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