The importance of tryptic-like activity in purified enzyme blends for efficient islet isolation.

Abstract

BACKGROUND The isolation of islets from the human pancreas critically depends on an efficient enzyme blend. Previous studies have solely focused on the presence of collagenase and neutral protease/thermolysin. Despite improved characterization of these components, the lot-related variability in efficacy still persists suggesting that additional so far… (More)
DOI: 10.1097/TP.0b013e31819499f0

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