The importance of methanogens associated with ciliate protozoa in ruminal methane production in vitro.

@article{Newbold1995TheIO,
  title={The importance of methanogens associated with ciliate protozoa in ruminal methane production in vitro.},
  author={Charles James Newbold and Bernadette Lassalas and J. P. Jouany},
  journal={Letters in applied microbiology},
  year={1995},
  volume={21 4},
  pages={230-4}
}
The importance of methanogenic bacteria associated with ciliate protozoa was estimated either by removing protozoa from whole rumen fluid (using defaunated rumen fluid to correct for the effects of centrifugation on bacteria) or by isolating the protozoa. Rumen fluid was withdrawn from sheep inoculated with either Polyplastron multivesiculatum, a co-culture of Isotricha prostoma plus Entodinium spp. or a mixed type B fauna of Entodinium, Eudiplodinium and Epidinium spp. Methanogenesis was… CONTINUE READING
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