The importance of hydration and DNA conformation in interpreting infrared spectra of cells and tissues.

@article{Wood2016TheIO,
  title={The importance of hydration and DNA conformation in interpreting infrared spectra of cells and tissues.},
  author={Bayden R. Wood},
  journal={Chemical Society reviews},
  year={2016},
  volume={45 7},
  pages={
          1980-98
        }
}
  • B. Wood
  • Published 29 March 2016
  • Chemistry, Biology
  • Chemical Society reviews
Since Watson and Crick's historical papers on the structure and function of DNA based on Rosalind Franklin's and Maurice Wilkin's X-ray diffraction patterns tremendous scientific curiosity has been aroused by the unique and dynamic structure of the molecule of life. A-DNA and B-DNA represent different conformations of the DNA molecule, which is stabilised by hydrogen interactions between base pairs, stacking interactions between neighboring bases and long-range intra- and inter-backbone forces… 

Tables from this paper

Probing the Hydrogen-Bonding Environment of Individual Bases in DNA Duplexes with Isotope-Edited Infrared Spectroscopy

A method using isotope editing and infrared (IR) spectroscopy to isolate IR signals from the thymine (T) C2=O carbonyl, consistent with the existence of a third, noncanonical CH···O H-bond between adenine and thymine in both Watson–Crick and Hoogsteen base pairs in DNA.

DNA structure change induced by guanosine radicals – a theoretical and spectroscopic study

A pathway of the formation of DNA damage caused by protons is established and molecular modifications of the DNA sugar-backbone affected by guanine radicals formed upon radiation exposure are explained, which lead to spectral changes between spectra of control and irradiated DNA.

Conformational Transitions of Double-Stranded DNA in Thin Films

Conformational transitions of double-stranded DNA in different environments have long been studied as vital parts of both in vitro and in vivo processes. In this study, utilizing Fourier transform

Long-Range DNA-Water Interactions.

Multivariate Analysis as a Tool for Quantification of Conformational Transitions in DNA Thin Films

The double-stranded B-form and A-form have long been considered the two most important native forms of DNA, each with its own distinct biological roles and hence the focus of many areas of study,

Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy

The working principles of AFM modes allowing high-resolution visualization of DNA structure under native (liquid) environment are described, indicating that AFM provides well-resolved structure of molecules at nanoscale, but does not reveal the chemical structure and composition of studied samples.

Nanomechanics of DNA-ligand interaction investigated with magnetic tweezers

A rational design for complexes that target two neighboring phosphates of the DNA backbone by molecular recognition resulting in a family of dinuclear complexes based on 2,7-disubstituted 1,8-naphthalenediol, which leads to a blocking of DNA synthesis as studied by polymerase chain reactions and cytotoxicity for human cancer cells.

Molecular Spectroscopic Markers of DNA Damage

All major spectral markers of DNA damage are presented in several tables, to give the reader a possibility of fast identification of the spectral signature related to a particular type ofDNA damage.

Contribution of Ribonucleic Acid (RNA) to the Fourier Transform Infrared (FTIR) Spectrum of Eukaryotic Cells.

It is proved that the spectral contribution centered at ∼1120 cm-1 is the most reliable indicator of variations in cellular RNA levels, that better correlates with cellular metabolic activity.
...

References

SHOWING 1-10 OF 240 REFERENCES

Infrared spectroscopy of DNA.

Circular dichroism and conformational polymorphism of DNA

Here the authors review studies that provided important information about conformational properties of DNA using circular dichroic (CD) spectroscopy, which significantly participated in all basic conformational findings on DNA.

Detection of an en masse and reversible B- to A-DNA conformational transition in prokaryotes in response to desiccation

This work reports the first direct observation of a fully reversible en masse conformational transition between B- and A-DNA within live bacterial cells using Fourier transform infrared (FTIR) spectroscopy.

Monitoring the reversible B to A-like transition of DNA in eukaryotic cells using Fourier transform infrared spectroscopy

It is reported that a B- to A-like DNA conformational change can occur in the nuclei of intact cells in response to dehydration, and this transition is reversible upon rehydration in air-dried cells.

Infrared Studies on the Backbone Conformation of Nucleic Acids

The presence and assignment of low frequency infrared bands characteristic of the A, B and C forms of DNA (Salmon Sperm and Micrococcus Lysodeikticus) and of synthetic poly (dA-T): (dA-T) and of

DNA Structure and Function

This chapter discusses the structure and function of DNA, which is a right handed helix of two individual antiparallel DNA strands that is the source of all intrinsic genetic information.

Spectroscopic analysis of the interaction of lomustine with calf thymus DNA.

IR spectroscopic studies of major cellular components. III. Hydration of protein, nucleic acid, and phospholipid films.

The IR absorption spectra of protein, DNA, RNA, and phospholipid films as a function of the water content are reported. We find that the hydration of protein films affects the peak intensity of amide
...