The importance of homologous recombination in the generation of large deletions in hybrid plasmids in Amycolatopsis mediterranei.

@article{Tuteja2000TheIO,
  title={The importance of homologous recombination in the generation of large deletions in hybrid plasmids in Amycolatopsis mediterranei.},
  author={Deepak Tuteja and Mikhael Dua and Rita Khanna and Nidhi Dhingra and Monisha Khanna and Harsimranjeet Kaur and D. M. Saxena and Rup Lal},
  journal={Plasmid},
  year={2000},
  volume={43 1},
  pages={
          1-11
        }
}
  • Deepak Tuteja, Mikhael Dua, +5 authors Rup Lal
  • Published in Plasmid 2000
  • Biology, Medicine
  • The cloning vector pRL60 was developed previously as a tool for genetic manipulations in Amycolatopsis mediterranei, which produces the commercially and medicinally important antibiotic rifamycin. Here, a method based on intraplasmid recombinations is described for the construction of smaller plasmids in A. mediterranei, which also helped in delimiting the origin of replication (pA-rep) of the parent plasmid. The strategy involved the cloning of a selectable marker, erythromycin resistance gene… CONTINUE READING

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