The impact of gene knock-down and vaccination against salivary metalloproteases on blood feeding and egg laying by Ixodes ricinus.

@article{Decrem2008TheIO,
  title={The impact of gene knock-down and vaccination against salivary metalloproteases on blood feeding and egg laying by Ixodes ricinus.},
  author={Yves Decrem and Marcel Mariller and Kathia Lahaye and Virginie Blasioli and J{\'e}r{\^o}me Beaufays and Karim Zouaoui Boudjeltia and Michel Vanhaeverbeek and Martine C{\'e}rutti and Michel Brossard and Luc Vanhamme and Edmond Godfroid},
  journal={International journal for parasitology},
  year={2008},
  volume={38 5},
  pages={549-60}
}
Two cDNAs coding homologous putative metalloproteases (Metis 1 and Metis 2, expected molecular weights of 55.6 and 56.0kDa, respectively) were identified from the hard tick Ixodes ricinus. The expression of Metis genes was induced in salivary glands during tick blood meal. RNA interference was used to assess the role of both Metis 1 and Metis 2 in tick feeding. It was found that salivary gland extracts lacking Metis 1-2 had a restricted ability to interfere with fibrinolysis. RNAi against Metis… CONTINUE READING