The impact of Nucleofection® on the activation state of primary human CD4 T cells.

@article{Zhang2014TheIO,
  title={The impact of Nucleofection® on the activation state of primary human CD4 T cells.},
  author={Mingce Zhang and Zhengyu Ma and Nithianandan Selliah and Greta E. Weiss and Anna Genin and Terri Helman Finkel and Randy Quentin Cron},
  journal={Journal of immunological methods},
  year={2014},
  volume={408},
  pages={123-31}
}
Gene transfer into primary human CD4 T lymphocytes is a critical tool in studying the mechanism of T cell-dependent immune responses and human immunodeficiency virus-1 (HIV-1) infection. Nucleofection® is an electroporation technique that allows efficient gene transfer into primary human CD4 T cells that are notoriously resistant to traditional electroporation. Despite its popularity in immunological research, careful characterization of its impact on the physiology of CD4 T cells has not been… CONTINUE READING