The immunochemical detection and quantitation of intracellular ubiquitin-protein conjugates.

@article{Haas1985TheID,
  title={The immunochemical detection and quantitation of intracellular ubiquitin-protein conjugates.},
  author={Amy Louise Haas and Peter Bright},
  journal={The Journal of biological chemistry},
  year={1985},
  volume={260 23},
  pages={
          12464-73
        }
}
ATP, ubiquitin-dependent proteolysis proceeds through covalent intermediates between target proteins destined for degradation and the 8,600-Da polypeptide ubiquitin. The ubiquitin moiety therefore represents a sensitive immunological marker for the specificity and function of this novel post-translational modification. Methods are described for the immunochemical detection of ubiquitin conjugates immobilized on nitrocellulose filters following electrophoretic transfer from sodium dodecyl… CONTINUE READING

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