The identification of free-living environmental isolates of amoebae from Bulgaria

@article{Tsvetkova2003TheIO,
  title={The identification of free-living environmental isolates of amoebae from Bulgaria},
  author={N. G. Tsvetkova and Mark Schild and Stefan Panaiotov and Rossitza Kurdova-Mintcheva and Bruno Gottstein and Julia Walochnik and Horst Asp{\"o}ck and Mar Siles Lucas and Norbert M{\"u}ller},
  journal={Parasitology Research},
  year={2003},
  volume={92},
  pages={405-413}
}
A survey was carried out in Bulgaria to determine the presence of free-living amoebae (FLA) from environmental sources. In 171 (61.1%) of 280 samples, isolates of Acanthamoeba with group II or III morphology, as well as Hartmannella spp. were recovered. Five isolates named “6” (artificial lake), Ep (lake), G2 (soil), R4* (river) and PK (spring water)—all exhibiting a highly efficient proliferation in axenic cultures—were subsequently cloned and subjected to molecular analyses for identification… CONTINUE READING

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