The identification of catalytic pentad in the haloalkane dehalogenase DhmA from Mycobacterium avium N85: reaction mechanism and molecular evolution.

@article{Pavlova2007TheIO,
  title={The identification of catalytic pentad in the haloalkane dehalogenase DhmA from Mycobacterium avium N85: reaction mechanism and molecular evolution.},
  author={Martina Pavlová and Martin Klvaňa and Andrea Jesenská and Zbyněk Prokop and Hana Kone{\vc}n{\'a} and Takashi Sato and Masataka Tsuda and Yuji Nagata and Jiř{\'i} Damborsk{\'y}},
  journal={Journal of structural biology},
  year={2007},
  volume={157 2},
  pages={
          384-92
        }
}
Haloalkane dehalogenase DhmA from Mycobacterium avium N85 showed poor expression and low stability when produced in Escherichia coli. Here, we present expression DhmA in newly constructed pK4RP rhodococcal expression system in a soluble and stable form. Site-directed mutagenesis was used for the identification of a catalytic pentad, which makes up the reaction machinery of all currently known haloalkane dehalogenases. The putative catalytic triad Asp123, His279, Asp250 and the first halide… Expand
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