The identification and characterization of a urinary mutagen resulting from cigarette smoke.

@article{Connor1983TheIA,
  title={The identification and characterization of a urinary mutagen resulting from cigarette smoke.},
  author={Thomas H. Connor and V. M. Sadagopa Ramanujam and Jonathan B Ward and Marvin S. Legator},
  journal={Mutation research},
  year={1983},
  volume={113 2},
  pages={161-72}
}
The urine of a cigarette smoker who excretes exceptionally mutagenic urine was analyzed for several factors affecting mutagenicity. S. typhimurium strain TA98 was always more sensitive to XAD-2 urine concentrates than TA100. With TA98, as high as 85 revertants per ml of urine were produced. It was observed that incubation with beta-glucuronidase was not required for expression of mutagenicity but that a complete S9 mix was needed to convert the material in the concentrate to the ultimate… CONTINUE READING