The hyaluronate synthase from a eukaryotic cell line.

  title={The hyaluronate synthase from a eukaryotic cell line.},
  author={Ludger Klewes and Eva Ann Turley and Peter Prehm},
  journal={The Biochemical journal},
  volume={290 ( Pt 3)},
The hyaluronate synthase complex was identified in plasma membranes from B6 cells. It contained two subunits of molecular masses 52 kDa and 60 kDa which bound the precursor UDP-GlcA in digitonin solution and partitioned into the aqueous phase, together with nascent hyaluronate upon Triton X-114 phase separation. The 52 kDa protein cross-reacted with poly- and monoclonal antibodies raised against the streptococcal hyaluronate synthase and the 60 kDa protein was recognized by monoclonal… CONTINUE READING