The hyaluronate lyase of Staphylococcus aureus - a virulence factor?

@article{Makris2004TheHL,
  title={The hyaluronate lyase of Staphylococcus aureus - a virulence factor?},
  author={George Makris and John D Wright and Eileen Ingham and K. Todd Holland},
  journal={Microbiology},
  year={2004},
  volume={150 Pt 6},
  pages={2005-13}
}
The hyaluronate lyase (HL) gene of Staphylococcus aureus 8325-4 (hysA) was inactivated in vitro with the insertion of the erythromycin determinant, ermC, from plasmid pE194. The hysA : : ermC mutation was introduced into S. aureus via a temperature-sensitive shuttle vector, where it underwent homologous recombination with the wild-type (w.t.) allele. The insertion of ermC in the chromosomal hysA locus was confirmed by Southern blot hybridization and the loss of HL activity was demonstrated… CONTINUE READING