The human interleukin-2 receptor beta-chain gene: genomic organization, promoter analysis and chromosomal assignment

Abstract

The chromosomal gene for the human interleukin-2 receptor beta-chain (IL-2R beta) was isolated and characterized. The entire IL-2R beta gene is composed of ten exons spanning about 24.3 kilobases, in which the protein is encoded by the exons 2-10. The cysteine rich extracellular region which displays a significant evolutionary resemblance to other cytokine receptors, as well as growth hormone and prolactin receptors, is encoded primarily by exons 3 and 4, whereas the membrane proximal, cysteine poor domain showing a homology with type III modules of fibronectin is encoded by exon 7. Sequence analysis of the 5'-flanking region revealed the presence of potential binding sites for transcription factors such as Octamer binding factors, AP-1, AP-2 as well as the 'GC-clusters'. At least five potential cap sites were identified by S1 mapping analysis. The 850 bp DNA sequence of the 5'-flanking region exhibited constitutive promoter activity when it was linked upstream of the HSV-tk reporter gene and then transfected into YT cells, a human leukemic cell line. By applying the RFLP linkage analysis, the IL-2R beta gene has been assigned to chromosome 22q12-13.

DOI: 10.1093/nar/18.13.3697

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@article{Shibuya1990TheHI, title={The human interleukin-2 receptor beta-chain gene: genomic organization, promoter analysis and chromosomal assignment}, author={H. Shibuya and M. Yoneyama and Y. Nakamura and H. Harada and M. Hatakeyama and S. Minamoto and T. Kono and T. Doi and R. White and T. Taniguchi}, journal={Nucleic acids research}, year={1990}, volume={18 13}, pages={3697-703} }