The human insulin receptor cDNA: The structural basis for hormone-activated transmembrane signalling

  title={The human insulin receptor cDNA: The structural basis for hormone-activated transmembrane signalling},
  author={Yousuke Ebina and Leland Ellis and Kurt Jarnagin and Marc Edery and László Gráf and Eric Clauser and Jing-hsiung James Ou and Frank R. Masiarz and Yuet Wai Kan and I. D. Goldfine and Richard A. Roth and William J. Rutter},

The human insulin receptor cDNA: a new tool to study the function of this receptor.

An expression system of the hIR cDNA in eucaryotic cells and site-directed mutagenesis experiments show that the C terminal 112 amino acid portion of the beta subunit is important for the stability of this protein.

Molecular and Cellular Biology of Insulin‐receptor Internalization a

The insulin receptor is a member of the family of receptor tyrosine kinases and consists of two external a subunits and two transmembrane 6 subunits linked by disulfide bonds in an a2p2

Truncation of the ectodomain of the human insulin receptor results in secretion of a soluble insulin binding protein from transfected CHO cells

Results with a soluble derivative of the hIR cytoplasmic protein/tyrosine kinase domain are consistent with the deduced topology, and the IR is indeed comprised of two large soluble domains connected by a single membrane spanning domain.

Insulin-Receptor Approaches to Studying Protein Kinase Domain

Evidence suggests that phosphorylation of tyrosyl residues 1146, 1150, and 1151 in the kinase domain of the β-subunit play a role in activation of the insulin-insulin-receptor system and point mutations in the cytoplasmic portion indicate that additional sites are important for receptor function.

Identification of a ligand-binding region of the human insulin receptor encoded by the second exon of the gene.

Structure-function studies of the insulin molecule indicate that an insulin B chain domain comprising residues 22-26 is involved both in binding to the insulin receptor (INSR) and in insulin dimer

Structure and ligand specificity of the Drosophila melanogaster insulin receptor

The insulin-binding and protein tyrosine kinase subunits of the Drosophila melanogaster insulin receptor homolog have been identified and characterized by using antipeptide antibodies elicited to the

The rat insulin receptor: primary structure and conservation of tissue-specific alternative messenger RNA splicing.

The splicing pattern of sequences homologous to Exon 11 was found to be highly conserved, providing further evidence that these two forms of the insulin receptor may serve important functional or regulatory roles.

Human Skeletal Muscle Insulin Receptor Substrate-1: Characterization of the cDNA, Gene, and Chromosomal Localization

The human IRS-1 cDNA is cloned from human skeletal muscle, localized and cloned the human taxonomic receptor substrate-1 gene, and studied the expression of the protein in Chinese hamster ovary cells, indicating that IRS- 1 is widely expressed and highly conserved across species and tissues.



Autophosphorylation sites on the epidermal growth factor receptor

It is reported here that three tyrosine sites near the C-terminus are phosphorylated in vitro in the human EGF receptor, and this site is absent in the v-erb-B protein of avian erythroblastosis virus (AEV), which may influence tyosine kinase activity.

Insulin receptor: structure and function.

After binding to cell surface receptors, insulin, along with its receptor, is internalized by an endocytic process, which in contrast to multiplication-stimulating activity is affinity cross-linked to a protein with an entirely different subunit structure.

Nucleotide sequence of a cDNA clone encoding human preproinsulin

The cloning of a cDNA prepared from human insulin mRNA and an analysis of the nucleotide sequence of the cloned molecule including the region coding for the prepeptide and portions of the 5′- and the 3′-untranslated regions of the molecule are reported.

Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells

The complete 1,210-amino acid sequence of the human epidermal growth factor (EGF) receptor precursor, deduced from cDNA clones derived from placental and A431 carcinoma cells, reveals close

Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences

Each of six peptides derived from the human epidermal growth factor (EGF) receptor very closely matches a part of the deduced sequence of the v-erb-B transforming protein of avian erythroblastosis

Assignment of the human gene for the low density lipoprotein receptor to chromosome 19: synteny of a receptor, a ligand, and a genetic disease.

The availability of a species-specific monoclonal antibody that recognizes the low density lipoprotein (LDL) receptor of human but not hamster origin permitted assignment of the structural gene for the human receptor to chromosome 19, raising the possibility of an evolutionary link between a protein ligand and its receptor.