• Corpus ID: 28170850

The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan.

@article{Badcock1999TheHE,
  title={The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan.},
  author={G. G. Badcock and Christine Pigott and J. R. Goepel and Peter W. Andrews},
  journal={Cancer research},
  year={1999},
  volume={59 18},
  pages={
          4715-9
        }
}
Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and alpha-fetoprotein, especially in those patients… 

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References

SHOWING 1-10 OF 45 REFERENCES
Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells.
TLDR
The epitopes defined by the present antibodies differ from those recognized by the other human EC cell-specific monoclonal antibodies that have been described and provide new markers for studying the differentiation of pluripotent human EC cells.
High-molecular-weight glycoproteins of human teratocarcinoma defined by monoclonal antibodies to carbohydrate determinants.
TLDR
Three mouse monoclonal antibodies to distinct cell surface antigens were derived from immunizations with cells of Tera-1, a human teratocarcinoma cell line, and a membrane preparation of placental tissue, finding expression in normal tissues is restricted to the epithelium of fetal intestine and bronchus.
Cell‐surface antigens of a clonal human embryonal carcinoma cell line: Morphological and antigenic differentiation in culture
TLDR
This well‐characterized system of human EC cells provides a model for the future investigation of other human teratocarcinoma cell lines and for the analysis of cellular differentiation during early human development.
A novel keratan sulphate proteoglycan from a human embryonal carcinoma cell line.
TLDR
The biochemical nature of the molecule described here, along with tissue distribution studies using GCTM-2, indicates that the antigen is not related to previously described keratan sulphate proteoglycans.
Differentiation antigens of human germ cell tumours: distribution of carbohydrate epitopes on glycolipids and glycoproteins analyzed using PDMP, an inhibitor of glycolipid synthesis.
TLDR
Resistance to PDMP inhibition suggests that glycoproteins carry the immunodominant form of SSEA1 on the cell surface of differentiated human EC cells and undifferentiated murine EC cells.
Comparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours
The pattern of cell surface antigen expression of a set of cell lines derived from human germ cell tumours and corresponding to various cell phenotypes found within these tumours was studied using
Cell line derived from a metastasis of a human testicular germ cell tumor.
A cell line, designated 833K-E, has been established from a metastasis of a human testicular germ cell tumor that consisted of four histological types of tumor cells. The 833K-E cells have
High-molecular-weight glycoproteins are the major carriers of the carbohydrate differentiation antigens I, i and SSEA-1 of mouse teratocarcinoma cells.
TLDR
Observations indicate that glycoproteins rather than glycolipids are the major carriers of carbohydrate differentiation antigens I, i and SSEA-1 in the teratocarcinoma cell lines.
The surface antigen phenotype of human embryonal carcinoma cells: modulation upon differentiation and viral infection.
TLDR
The shedding of certain surface antigens characteristic of EC cells and their detection in the serum of patients could provide a useful additional means of monitoring the progress of therapy and provide the basis for improved histological classification of human germ cell tumors.
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