The human elongation factor 1 alpha (EF-1 alpha) first intron highly enhances expression of foreign genes from the murine cytomegalovirus promoter.

@article{Kim2002TheHE,
  title={The human elongation factor 1 alpha (EF-1 alpha) first intron highly enhances expression of foreign genes from the murine cytomegalovirus promoter.},
  author={Seon-Young Kim and J Lee and Hyun-Seock Shin and Hye-Ja Kang and Yeon-soo Kim},
  journal={Journal of biotechnology},
  year={2002},
  volume={93 2},
  pages={183-7}
}
In order to develop a highly efficient mammalian expression vector, we constructed a vector by the combination of the murine cytomegalovirus (MCMV) immediate early (IE) promoter and the human elongation factor one alpha (EF-1 alpha) first intron. The MCMV IE promoter was several fold stronger than the human cytomegalovirus (HCMV) immediate early (IE) promoter and the human elongation factor one alpha (EF-1 alpha) promoter in various mammalian cell lines such as NIH3T3, Neuro-2a, 293T or HT1080… CONTINUE READING

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