The human combinatorial antibody library HuCAL GOLD combines diversification of all six CDRs according to the natural immune system with a novel display method for efficient selection of high-affinity antibodies.

@article{Rothe2008TheHC,
  title={The human combinatorial antibody library HuCAL GOLD combines diversification of all six CDRs according to the natural immune system with a novel display method for efficient selection of high-affinity antibodies.},
  author={Christine Rothe and Stefanie Urlinger and Corinna L{\"o}hning and Josef Prassler and Yvonne Stark and Ute J{\"a}ger and Bernd Hubner and Michael Bardroff and Ingrid Pradel and Melanie Boss and Renate Bittlingmaier and Tschimegma Bataa and Christian Frisch and Bodo Brocks and Annemarie Honegger and Margit Urban},
  journal={Journal of molecular biology},
  year={2008},
  volume={376 4},
  pages={1182-200}
}
This article describes the generation of the Human Combinatorial Antibody Library HuCAL GOLD. HuCAL GOLD is a synthetic human Fab library based on the HuCAL concept with all six complementarity-determining regions (CDRs) diversified according to the sequence and length variability of naturally rearranged human antibodies. The human antibody repertoire was analyzed in-depth, and individual CDR libraries were designed and generated for each CDR and each antibody family. Trinucleotide mixtures… CONTINUE READING