The human Rad51 K133A mutant is functional for DNA double-strand break repair in human cells.

@article{Forget2007TheHR,
  title={The human Rad51 K133A mutant is functional for DNA double-strand break repair in human cells.},
  author={Anthony L. Forget and Matthew S. Loftus and Dharia A McGrew and Brian T. Bennett and Kendall L. Knight},
  journal={Biochemistry},
  year={2007},
  volume={46 11},
  pages={3566-75}
}
The human Rad51 protein requires ATP for the catalysis of DNA strand exchange, as do all Rad51 and RecA-like recombinases. However, understanding the specific mechanistic requirements for ATP binding and hydrolysis has been complicated by the fact that ATP appears to have distinctly different effects on the functional properties of human Rad51 versus yeast Rad51 and bacterial RecA. Here we use RNAi methods to test the function of two ATP binding site mutants, K133R and K133A, in human cells… CONTINUE READING

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