The human PRD-like homeobox gene LEUTX has a central role in embryo genome activation

@article{Jouhilahti2016TheHP,
  title={The human PRD-like homeobox gene LEUTX has a central role in embryo genome activation},
  author={Eeva-Mari Jouhilahti and Elo Madissoon and Lise Vesterlund and Virpi T{\"o}h{\"o}nen and Kaarel Krjut{\vs}kov and {\'A}lvaro Plaza Reyes and Sophie Petropoulos and Robert Månsson and Sten Linnarsson and Thomas R. B{\"u}rglin and Fredrik Lanner and Outi Hovatta and Shintaro Katayama and Juha Kere},
  journal={Development (Cambridge, England)},
  year={2016},
  volume={143},
  pages={3459 - 3469}
}
Leucine twenty homeobox (LEUTX) is a paired (PRD)-like homeobox gene that is expressed almost exclusively in human embryos during preimplantation development. We previously identified a novel transcription start site for the predicted human LEUTX gene based on the transcriptional analysis of human preimplantation embryos. The novel variant encodes a protein with a complete homeodomain. Here, we provide a detailed description of the molecular cloning of the complete homeodomain-containing LEUTX… 

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References

SHOWING 1-10 OF 68 REFERENCES
Novel PRD-like homeodomain transcription factors and retrotransposon elements in early human development
TLDR
High-resolution RNA sequencing of 348 oocytes, zygotes and single blastomeres from 2- to 3-day-old embryos provides a detailed analysis of the human preimplantation transcriptome and identifies significantly enriched gene regulatory motifs that often overlap with Alu elements.
Evolution of homeobox genes
  • P. Holland
  • Biology
    Wiley interdisciplinary reviews. Developmental biology
  • 2013
TLDR
Genomic data suggest that the ANTP class diversified by extensive tandem duplication to generate a large array of genes, including an NK gene cluster and a hypothetical ProtoHox gene cluster that duplicated to generate Hox and ParaHox genes.
The leucine twenty homeobox (LEUTX) gene, which lacks a histone acetyltransferase domain, is fused to KAT6A in therapy‐related acute myeloid leukemia with t(8;19)(p11;q13)
TLDR
The present study shows that the leucine twenty homeobox (LEUTX) gene on 19q13 is fused to the KAT6A gene on 8p11 in a therapy‐related AML with t(8;19)(p11;q13) using the cDNA bubble PCR method.
The zinc-finger protein Zelda is a key activator of the early zygotic genome in Drosophila
TLDR
The zinc-finger protein Zelda (Zld; Zinc-finger early Drosophila activator) binds specifically to these sites and is capable of activating transcription in transient transfection assays, suggesting that Zld may also regulate maternal RNA degradation during the maternal-to-zygotic transition.
The dynamics of vertebrate homeobox gene evolution: gain and loss of genes in mouse and human lineages
TLDR
There has been much more homeobox gene loss in the rodent evolutionary lineage than in the primate lineage, and a step towards relating genomic evolution to phenotypic evolution is taken.
Temporal regulation of a paired-like homeodomain repressor/TLE corepressor complex and a related activator is required for pituitary organogenesis.
TLDR
The data suggest that the opposing actions of two structurally-related DNA-binding paired-like homeodomain transcription factors, coordinate pituitary organogenesis by reciprocally repressing and activating target genes in a temporally specific fashion, on the basis of the actions of a critical, coexpressed TLE corepressor.
The TAGteam DNA motif controls the timing of Drosophila pre-blastoderm transcription
TLDR
This TAGteam dose effect shows that activators must also play an important role in the timing of pre-blastoderm gene expression, and suggests that CAGGTAG is not specific to early sex-determination genes, since it is over-represented upstream of most genes that are transcribed pre-cellular blastoderm, not just those involved in sex determination.
Homeodomain subtypes and functional diversity.
TLDR
Homeobox genes perform key roles for all aspects of the development of an organism, and several particular types of homeodomain proteins play roles in the determination of cell fates and cell differentiation.
Classification and nomenclature of all human homeobox genes
TLDR
A comprehensive survey of homeobox genes and pseudogenes in the human genome is conducted, many new loci are described, and the classification and nomenclature of homeOBox genes are revised.
...
...