The history of sperm cryopreservation

  title={The history of sperm cryopreservation},
  author={Eric M. Walters and James D. Benson and Erik J. Woods and John Kenneth Critser},
While there have been several relatively recent comprehensive reviews of mammalian sperm cryopreservation (Watson, 2001; Leibo, 2004), this chapter is not meant to be a review of the literature of mammalian sperm cryopreservation. Instead, we intend to provide an understanding of the history, current status, and potential future direction of mammalian sperm cryobiology. There are many reasons why sperm cryopreservation is important, including: (1) maintenance of genetic diversity in domestic… 

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Poultry semen cryopreservation technologies

This paper reviews the possible reasons for the lower success of poultry sperm cryopreservation and suggests that poultry sperm membranes contain higher quantities of polyunsaturated fatty acids than mammalian sperm, and hence may require more antioxidant protection.

Bovine sperm selection procedure prior to cryopreservation for improvement of post-thawed semen quality and fertility

These results provide solid evidence that improvement of post-thawed semen quality by SSRT method is beneficial in terms of cryosurvival, longevity of post -thawed sperm, and optimization of in vivo fertilization, embryo development and calving as supported by the favorable results of field fertility study.


The published techniques are not robust and require further development to improve the motility of rat sperm after cryopreservation and achieve pregnancy via intrauterine insemination.

Dry Preservation of Spermatozoa: Considerations for Different Species.

The range of technologies used to dehydrate sperm and the effect of processing and storage conditions on fertilization and embryogenesis using dried sperm are reviewed in the context of reproductive physiology and cellular morphology in different species.

Regulation of the ART Laboratory

The field of ART was established in the 1970s with successful in vitro fertilization and embryo transfer for the treatment of infertility and has expanded to include procedures and services for third-party reproduction, fertility preservation, and sex selection.

Possibility of computer experiment in study of animal spermatozoa heterogeneity

A simulation model for evaluating the survival and fertilizing capacity of animal spermatozoa was developed, taking into account the initial condition of the sperm and the effectiveness of cryopreservation stages, and showed that the difference between the values of initial motility and fertilization capacity of sperm varies from 50 to 100% depending on the difference of biological parameters.

Long‐Term Storing of Frozen Semen at −196°C does not Affect the Post-Thaw Sperm Quality of Bull Semen

The sperm quality of bovine semen is not affected by long‐ term storage at −196°C, and both parameters showed optimal values of sperm viability and total sperm motility.

Cryobanking of aquatic species.

The effect of progesterone on motility and the acrosome reaction in vitrified and cryopreserved human spermatozoa

The progesterone treatment after cryopreservation was able to significantly improve the motility parameters of sperm samples and it had an especially large impact on samples classed as teratozoospermic.

Development of a bovine sperm selection procedure for improvement of livestock fertility

livestock, Improving the reproductive performance of livestock has wide-ranging significance that includes promotion of local industry, bioeconomy, and stabilization of food supply. Our research



Cryopreservation of murine spermatozoa.

Success in deriving live young from intracytoplasmic sperm injection using sperm frozen under suboptimal conditions raises the possibility of using this technique for the ultimate rescue of sperm regardless of the success of cryopreservation, although this technique requires additional development and verification of its efficacy before it will be suitable for general laboratory use.

Semen cryopreservation: a genetic explanation for species and individual variation?

This review aims to explore the phenomenon of consistent variation in frozen semen quality between species and between individuals in an effort to find new insights into the reasons for cryoinjury.

Cryopreservation of sperm: indications, methods and results.

Sperm cryopreservation has revolutionized the field of assisted reproduction and provides hope for men undergoing chemotherapy, radiation or radical surgery who once had no chance for future fertility.

Simple and Efficient In Vitro Fertilization with Cryopreserved C57BL/6J Mouse Sperm1

  • M. Bath
  • Biology
    Biology of reproduction
  • 2003
Nonmotile sperm and cell debris were removed from thawed suspensions of C57BL/6J mouse sperm, and the remaining, largely progressively motile sperm were used for in vitro fertilization and the production of 2-cell embryos and the birth of live pups indicate that cryopreservation of sperm is a practical way to archive the haploid genome of genetically altered C57bl/ 6J mice.

Large numbers of mice established by in vitro fertilization with cryopreserved spermatozoa: implications and applications for genetic resource banks, mutagenesis screens, and mouse backcrosses

It is demonstrated that it is possible to establish efficient, comprehensive, and deep archives and that potentially thousands of offspring can be derived from the frozen spermatozoa of a single mutant male mouse.

Decrease of Fertilizing Ability of Mouse Spermatozoa after Freezing and Thawing Is Related to Cellular Injury1

The results of in vitro fertilization (IVF), scanning electron microscopy (SEM), and transmission electron microscopeopy (TEM) showed a strong correlation between the frequency of aberrant spermatozoa (FAS) and fertilization rates (FR; C57BL/6J: FAS, 83.7%; FR, 17.0%).

A novel approach to sperm cryopreservation.

It is shown that improved methods of cryopreservation may be developed by specifically manipulating the manner in which cells experience physical changes instead of imposing a linear temperature reduction.

Possibility of Long-Term Preservation of Freeze-Dried Mouse Spermatozoa1

Results indicate that the determination of accelerated degradation kinetics can be applied to the preservation of freeze-dried mouse spermatozoa, and appear to require being kept at lower than −80°C for long-term preservation.

Sperm cryopreservation for men with nonmalignant, systemic diseases: a descriptive study.

The results indicate that pretreatment semen quality in these patients is adequate for reproductive techniques, and it is believed that cryopreservation should be offered to patients of reproductive age with disease or treatment regimens that may cause infertility.