The histone chaperone ASF1 is essential for sexual development in the filamentous fungus Sordaria macrospora

  title={The histone chaperone ASF1 is essential for sexual development in the filamentous fungus Sordaria macrospora},
  author={Stefan Gesing and Daniel Schindler and Benjamin Fr{\"a}nzel and Dirk Wolters and Minou Nowrousian},
  journal={Molecular Microbiology},
Ascomycetes develop four major types of fruiting bodies that share a common ancestor, and a set of common core genes most likely controls this process. One way to identify such genes is to search for conserved expression patterns. We analysed microarray data of Fusarium graminearum and Sordaria macrospora, identifying 78 genes with similar expression patterns during fruiting body development. One of these genes was asf1 (anti‐silencing function 1), encoding a predicted histone chaperone. asf1… 
The transcription factor PRO44 and the histone chaperone ASF1 regulate distinct aspects of multicellular development in the filamentous fungus Sordaria macrospora
Describing the roles of the transcription factor PRO44 and the histone chaperone ASF1 in fruiting body development indicates distinct roles in the regulation of sexual development in fungi.
Functional Analysis of Developmentally Regulated Genes chs7 and sec22 in the Ascomycete Sordaria macrospora
The authors' data indicate that chs7 is dispensable for fruiting body formation in S. macrospora, whereas sec22 is required for ascospore maturation and germination and thus involved in late stages of sexual development.
Functional characterization of the developmental genes asm2, asm3, and spt3 required for fruiting body formation in the filamentous ascomycete Sordaria macrospora.
The formation of fruiting bodies is one of the most complex developmental processes in filamentous ascomycetes. It requires the development of sexual structures that give rise to meiosporangia (asci)
Functional Analysis of Mating Type Genes and Transcriptome Analysis during Fruiting Body Development of Botrytis cinerea
The development of fruiting bodies (“apothecia”) of the ubiquitous plant-pathogenic ascomycete Botrytis cinerea was studied and the first study ever of the transcriptome of pure ascospores in a filamentous fungus was provided.
Suppression subtractive hybridization and comparative expression analysis to identify developmentally regulated genes in filamentous fungi
Control studies with N. crassa orthologues of differentially regulated genes revealed a functional role during fruiting body development for the gene NCU05079, encoding a putative MFS peptide transporter.
Comparative Genomics and Transcriptomics To Analyze Fruiting Body Development in Filamentous Ascomycetes
Comparative transcriptomics indicated that gene expression patterns in developing fruiting bodies of the three species are more similar to each other than to nonsexual hyphae of the same species.
The Genome and Development-Dependent Transcriptomes of Pyronema confluens: A Window into Fungal Evolution
The genome was sequenced and transcriptomes assayed during development of Pyronema confluens, a saprobic Pezizomycete with a typical apothecium as fruiting body, to close the sequence gap among basal filamentous ascomycetes and allow conclusions about the evolution of fungal development.
Sordaria macrospora: 25 years as a model organism for studying the molecular mechanisms of fruiting body development
This review gives a brief overview of major method developments for S. macrospora, and focuses on recent results characterizing different processes involved in regulating development including several regulatory protein complexes, autophagy, transcriptional and chromatin regulation, and RNA editing.
PRO40 Is a Scaffold Protein of the Cell Wall Integrity Pathway, Linking the MAP Kinase Module to the Upstream Activator Protein Kinase C
The results imply that PRO40 is a scaffold protein for the CWI pathway, linking the MAPK module to the upstream activator PKC1, and the PRO40 N-terminal disordered region and the central region encompassing a WW interaction domain are sufficient to govern interaction with MEK1.


The gene for a lectin-like protein is transcriptionally activated during sexual development, but is not essential for fruiting body formation in the filamentous fungus Sordaria macrospora
The tap1 transcript is strongly upregulated during sexual development in Sordaria macrospora; however, analysis of a tap1 knockout strain shows that tap1 is not essential for fruiting body formation in S. macrospora.
The pro1(+) gene from Sordaria macrospora encodes a C6 zinc finger transcription factor required for fruiting body development.
The pro1(+) gene in fruiting body development was confirmed by trying to complement the mutant phenotype with in vitro mutagenized and truncated versions of the pro1 open reading frame, and Southern hybridization experiments indicated that pro 1(+) homologues are present in other sexually propagating filamentous ascomycetes.
The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora
Analysis of the sterile mutant pro41 that is unable to produce mature fruiting bodies and microarray analysis of gene expression in the mutants pro1, pro41 and the pro1/41 double mutant showed that pro41 is partly epistatic to pro1.
Microarray and real-time PCR analyses reveal mating type-dependent gene expression in a homothallic fungus
To analyze the molecular basis of homothallism and the role of mating products during fruiting body development, the mating type gene Smta-1 is deleted, encoding a high-mobility group domain (HMG) protein.
Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development.
It is reported that both AtASF1A and Atasf1B proteins bind histone H3, and are localized in the cytoplasm and the nucleus, establishing crucial roles for the AtASf1Aand AtASFs genes in chromatin replication, maintenance of genome integrity and cell proliferation during plant development.
The WW Domain Protein PRO40 Is Required for Fungal Fertility and Associates with Woronin Bodies
It is shown that the sterile pro40 mutant is defective in a 120-kDa WW domain protein that plays a pivotal role in fruiting body maturation of the homothallic ascomycete Sordaria macrospora.
A WD40 Repeat Protein Regulates Fungal Cell Differentiation and Can Be Replaced Functionally by the Mammalian Homologue Striatin
The data strongly suggest that an evolutionarily conserved cellular process controlling eukaryotic cell differentiation may regulate fruiting body formation.
A novel polyketide biosynthesis gene cluster is involved in fruiting body morphogenesis in the filamentous fungi Sordaria macrospora and Neurospora crassa
Analysis of microarray data revealed a gene cluster strongly upregulated during fruiting body development in the ascomycete Sordaria macrospora, and real time PCR analysis showed that the genes from the orthologous cluster in Neurospora crassa are also up regulated during development.
A Sordaria macrospora mutant lacking the leu1 gene shows a developmental arrest during fruiting body formation
  • U. Kück
  • Biology
    Molecular Genetics and Genomics
  • 2005
Analysis of mutant pro4 of the ascomycete Sordaria macrospora and expression data indicate that cross-pathway control is superimposed on leucine-specific regulation of fruiting body development in the leu1 mutant, which represents a novel mutant type in S. macrospora.
A homologue of the human STRIPAK complex controls sexual development in fungi
In S.’macrospora, truncation of PRO11 and PRO22 leads to distinct defects in sexual development and cell fusion, indicating a role for the fungal STRIPAK complex in both processes.