The histochemical application of dansylhydrazine as a fluorescent labeling reagent for sialic acid in cellular glycoconjugates

@article{Weber1975TheHA,
  title={The histochemical application of dansylhydrazine as a fluorescent labeling reagent for sialic acid in cellular glycoconjugates},
  author={Peter Weber and Frederick W. Harrison and Liselotte Hof},
  journal={Histochemistry},
  year={1975},
  volume={45},
  pages={271-277}
}
A new method for the fluorescent staining of sialic acid—containing glycoconjugates in fixed tissues is described. The procedure uses mild periodate oxidation, followed by condensation with dansylhydrazine and reduction of the hydrazones to hydrazines. The specificity of the reaction for sialic acid is tested on model glycoconjugates. The procedure gives superior resolution in comparison to the standard periodate Schiff procedure for cellular carbohydrates. 
9 Citations
14 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-9 of 9 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 14 references

Introduction of a fluorescent label into the carbohydrate moiety of glyeoeonjugates

  • P. Weber, L. I-Iof
  • Bioehem. biophys. Res. Commun. gS,
  • 1975

The topographical distribution of complex carbohydrates in the erythroeyte membrane

  • T. L. Steek, G. Dawson
  • J. biol. Chem
  • 1974

l~luoreseent-labelled aprotinin: A new reagent for the histoehemical detection of acid mueosubstanees

  • Kiernan, .A J, Stoddart, W g.
  • Histoehemie 114,
  • 1973

N - Aeetylneuraminie acid analogues . II . The action of Nacetylneuraminie acid aldolase on 8carbon and 7carbon analogues

  • M. Suttajit, J. Beidler
  • J . biol . Chem .
  • 1971

The gaschromatographic properties of biologically important Nacetylglueosamine derivatives , mono, diM , tri, tetra, and pentasaccharides

  • T. Bhatti
  • Biochim . biophys . Acta ( Amst . )
  • 1970

Wheatgerm agglutinin conjugated with fluorescein isothiocyanate staining of normal and transformed Chinese hamster cells

  • G C., S. I. Hakomori
  • Cytobios
  • 1970

Similar Papers

Loading similar papers…