The high-sensitivity determination of protein concentrations by the enhancement of Rayleigh light scattering of Arsenazo-DBN.

@article{Li2000TheHD,
  title={The high-sensitivity determination of protein concentrations by the enhancement of Rayleigh light scattering of Arsenazo-DBN.},
  author={Q. Li and X. Chen and H Zhang and C. X. Xue and S Liu and Z D Hu},
  journal={The Analyst},
  year={2000},
  volume={125 8},
  pages={
          1483-6
        }
}
A new Rayleigh light scattering (RLS) assay of protein is presented in this paper. At the optimum pH 4.10, the weak RLS of Arsenazo-DBN can be greatly enhanced by the addition of proteins due to the interaction between protein and Arsenazo-DBN. Based on this, the reactions of Arsenazo-DBN and proteins, including bovine serum albumin, human serum album, gamma-globulin, egg albumin, lysozyme and trypsin, were studied. A new quantitative determination method for proteins has been developed. The… 
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Quantitative determination of proteins by the Rayleigh light-scattering technique after optimization of the derivation reaction with Arsenazo-DBS
RAYLEIGH LIGHT SCATTERING ENHANCING REACTION OF DIBROMOCHLOROARSENAZO AND PROTEINS SYSTEM AND ITS ANALYTICAL APPLICATION
Based on the enhancement effects of Rayleigh light scattering (RLS) on Arsenazo-DBC, a novel quantitative method for the determination of proteins in aqueous solutions has been proposed. The reaction…
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It is considered that the synergistic resonance caused by the absorption of both protein and SDBS could produce strong RLS, while absorption of protein only in the SLS system could cause relatively weak RLS.
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