The heterogeneity of the neuronal distribution of exogenous noradrenaline in the rat vas deferens

@article{Schmig2004TheHO,
  title={The heterogeneity of the neuronal distribution of exogenous noradrenaline in the rat vas deferens},
  author={Edgar Sch{\"o}mig and C. -L. Sch{\"o}nfeld and Thomas Halbr{\"u}gge and Karl Heinz Graefe and Ullrich Trendelenburg},
  journal={Naunyn-Schmiedeberg's Archives of Pharmacology},
  year={2004},
  volume={342},
  pages={160-170}
}
SummaryAfter loading of the incubated rat vas deferens with 0.2 μmol/l 3H-noradrenaline (followed by 100 min of wash-out with amine-free solution), the efflux of endogenous and exogenous compounds was determined by HPLC with electrochemical detection and by column chromatography with scintillation counting. Two different types of heterogeneity of labelling were found. The first one is due to the preferential labelling of varicosities close to the surface of the tissue, the second one to the… 
Autoradiographic study of the rat vas deferens incubated with 3H-noradrenaline
TLDR
Rat vasa deferentia were incubated with 0.2 μmol/l 3H-noradrenaline for 60 min and then washed out with amine-free solution for 100 min, revealing a preferential labelling of the varicosities in the immediate vicinity of the surface of the tissue.
A comparative study of the distribution of tritiated and endogenous noradrenaline in the rat vas deferens and in the dog spleen capsule
TLDR
It is concluded that the morphological characteristics of the tissues contribute to the differences in 3H-noradrenaline distribution in the adrenergic varicosities of these preparations.
The steady-state concentration gradient for 3H-noradrenaline generated by uptake1 in the extracellular space of the rat vas deferens incubated with this amine
TLDR
The rat vas deferens was incubated with 0.2 μmol/l 3H-noradrenaline for 60 min, washed out with amine-free solution for 100 min and then prepared for autoradiography, and grain density was determined as a function of the distance from the surface of the tissue.
The influence of the density of adrenergic innervation on the extracellular steady-state concentration gradient for 3H-noradrenaline
TLDR
The results show that the extracellular steady-state concentration gradient for 3H-noradrenaline (generated by uptake, during the incubation with this amine) largely depends on the density of the adrenergic innervation.
Differential distribution in, and release from, sympathetic nerve endings of endogenous noradrenaline and recently incorporated catecholamines
TLDR
It is concluded that recently incorporated amines are preferentially stored in varicosities close to the surface of the tissue and, in comparison with endogenous noradrenaline, are Preferentially released from sympathetically innervated organs.
The energy requirements for the basal efflux of 3H-noradrenaline from sympathetically innervated organs
TLDR
It is proposed that the greater contribution by outward transport of 3H-noradrenaline to spontaneous efflux in atria than in vasa deferentia does not reflect any basic difference between the varicosities in two different organs.
The sensitivity of adrenergic varicosities to the 3H-noradrenaline-releasing effect of potassium
TLDR
It is suggested that “organ differences” with respect to the sensitivity to high K+ may well be due to hypoxia of those varicosities that had been loaded with 3H-noradrenaline, which appears to be greater in incubated than in perfused organs and in the former it is greater in sparsely than in densely innervated tissues.
Carrier-mediated outward transport of dopamine from adrenergic varicosities of the vas deferens of reserpine-pretreated rats
TLDR
It is concluded that, after depletion of the noradrenaline stores by pretreatment with reserpine, an outward transport of axoplasmic dopamine is induced by the same mechanisms that are known to initiate an outward Transport of nor adrenaline.
Homogeneous or heterogeneous distribution of systemically administered adrenaline: organ dependence
TLDR
It is concluded that, even when the amine reaches the storage sites from the blood, it distributes homogeneously in the spleen capsule and heterogeneouslyIn the vas deferens, perhaps because there are more than one kind of storage vesicles in the vasdeferens.
Release and disposition of 3H-noradrenaline in the saphenous vein of neonate and adult dogs
TLDR
It is concluded that the evoked fractional release of 3H-noradrenaline is higher in Neonates than in adults because in neonates the majority of varicosities is situated closer to the surface of the tissue such that both uptake from the surrounding media into the stores and release from the stores into the surroundingmedia become easier in the neonates.
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References

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TLDR
Rat vasa deferentia were incubated with 0.2 μmol/l 3H-noradrenaline for 60 min and then washed out with amine-free solution for 100 min, revealing a preferential labelling of the varicosities in the immediate vicinity of the surface of the tissue.
The extent of neuronal re-uptake of 3H-noradrenaline in isolated vasa deferentia and atria of the rat
TLDR
Fractional rates were calculated on the basis of a mathematical model of the adrenergic nerve ending for unidirectional outward diffusion, for outward transport and for neuronal re-uptake for 3H-noradrenaline and found that the density of adrenergic innervation is lower in atria than in vasa deferentia.
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TLDR
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TLDR
It is concluded that, in the rabbit perfused heart, DOPEG is an important metabolite of endogenous noradrenaline, and DOMA is at best a minor product, either when the neurones are at rest or when nor adrenaline is released by sympathetic nerve stimulation, nicotine or tyramine.
Differential distribution in, and release from, sympathetic nerve endings of endogenous noradrenaline and recently incorporated catecholamines
TLDR
It is concluded that recently incorporated amines are preferentially stored in varicosities close to the surface of the tissue and, in comparison with endogenous noradrenaline, are Preferentially released from sympathetically innervated organs.
Stereoselectivity in the metabolism of 3H-noradrenaline during uptake into and efflux from the isolated rat vas deferens
TLDR
The results indicate that cocaine has only one site of action (neuronal uptake), while phenoxybenzamine exerts reserpine-like as well as cocaine-like effects.
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TLDR
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TLDR
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TLDR
Very high concentrations (especially of highly lipophilic substrates) were able to induce an additional intraneuronal release mechanism, presumably by increasing the pH inside storage vesicles.
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TLDR
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