The hemicholinium-3 sensitive high affinity choline transporter is internalized by clathrin-mediated endocytosis and is present in endosomes and synaptic vesicles.

@article{Ribeiro2003TheHS,
  title={The hemicholinium-3 sensitive high affinity choline transporter is internalized by clathrin-mediated endocytosis and is present in endosomes and synaptic vesicles.},
  author={Fabiola M. A. Ribeiro and Juliana Alves-Silva and Walter Volknandt and Cristina Martins-Silva and Hayat Mahmud and Alexander Wilhelm and Marcus Vin{\'i}cius Gomez and Rebecca Jane Rylett and Stephen S. G. Ferguson and Vania F Prado and Marco A M Prado},
  journal={Journal of neurochemistry},
  year={2003},
  volume={87 1},
  pages={136-46}
}
Synthesis of acetylcholine depends on the plasma membrane uptake of choline by a high affinity choline transporter (CHT1). Choline uptake is regulated by nerve impulses and trafficking of an intracellular pool of CHT1 to the plasma membrane may be important for this regulation. We have generated a hemagglutinin (HA) epitope tagged CHT1 to investigate the organelles involved with intracellular trafficking of this protein. Expression of CHT1-HA in HEK 293 cells establishes Na+-dependent… CONTINUE READING