The hU3-55K protein requires 15.5K binding to the box B/C motif as well as flanking RNA elements for its association with the U3 small nucleolar RNA in Vitro.

@article{Granneman2002TheHP,
  title={The hU3-55K protein requires 15.5K binding to the box B/C motif as well as flanking RNA elements for its association with the U3 small nucleolar RNA in Vitro.},
  author={Sander Granneman and Ger J. M. Pruijn and Wendy Horstman and Walther J. van Venrooij and R. Luhrmann and Nicholas James Watkins},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 50},
  pages={48490-500}
}
The 15.5K protein directly binds to the 5' stem-loop of the U4 small nuclear RNA, the small nucleolar (sno) RNA box C/D motif, and the U3 snoRNA-specific box B/C motif. The box B/C motif has also been shown to be essential for the association of the U3 small nucleolar ribonucleoprotein-specific protein hU3-55K. We therefore set out to determine how 15.5K and hU3-55K recognize the box B/C motif. By using an in vitro assembly assay, we show that hU3-55K effectively binds a sub-fragment of the U3… CONTINUE READING

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