The guanine-nucleotide-exchange factor BopE from Burkholderia pseudomallei adopts a compact version of the Salmonella SopE/SopE2 fold and undergoes a closed-to-open conformational change upon interaction with Cdc42.

@article{Upadhyay2008TheGF,
  title={The guanine-nucleotide-exchange factor BopE from Burkholderia pseudomallei adopts a compact version of the Salmonella SopE/SopE2 fold and undergoes a closed-to-open conformational change upon interaction with Cdc42.},
  author={Abhishek Kumar Upadhyay and Huan-Lin Wu and Christopher Williams and Terry Field and Edouard E. Galyov and Jean M. H. van den Elsen and Stefan Bagby},
  journal={The Biochemical journal},
  year={2008},
  volume={411 3},
  pages={
          485-93
        }
}
BopE is a type III secreted protein from Burkholderia pseudomallei, the aetiological agent of melioidosis, a severe emerging infection. BopE is a GEF (guanine-nucleotide-exchange factor) for the Rho GTPases Cdc42 (cell division cycle 42) and Rac1. We have determined the structure of BopE catalytic domain (amino acids 78-261) by NMR spectroscopy and it shows that BopE(78-261) comprises two three-helix bundles (alpha1alpha4alpha5 and alpha2alpha3alpha6). This fold is similar to that adopted by… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 16 CITATIONS

Type III Secretion in the Melioidosis Pathogen Burkholderia pseudomallei

VIEW 1 EXCERPT
CITES BACKGROUND

Aminopurine derivatives as putative SopE inhibitors.

Regulation of small GTPases by GEFs, GAPs, and GDIs.

VIEW 1 EXCERPT
CITES BACKGROUND

Subversion of cell signaling by pathogens.

VIEW 2 EXCERPTS
CITES BACKGROUND

References

Publications referenced by this paper.
SHOWING 1-10 OF 54 REFERENCES

Salmonella exchange factor SopE

  • I. Fricke, A. Scrima, A. Berken, A. Wittinghofer
  • Meth. Enzymol
  • 2007

The type III needle and the damage done.