The guanine-nucleotide-exchange factor BopE from Burkholderia pseudomallei adopts a compact version of the Salmonella SopE/SopE2 fold and undergoes a closed-to-open conformational change upon interaction with Cdc42.

@article{Upadhyay2008TheGF,
  title={The guanine-nucleotide-exchange factor BopE from Burkholderia pseudomallei adopts a compact version of the Salmonella SopE/SopE2 fold and undergoes a closed-to-open conformational change upon interaction with Cdc42.},
  author={A. Upadhyay and H. Wu and C. Williams and Terry Field and E. Galyov and J. van den Elsen and S. Bagby},
  journal={The Biochemical journal},
  year={2008},
  volume={411 3},
  pages={
          485-93
        }
}
  • A. Upadhyay, H. Wu, +4 authors S. Bagby
  • Published 2008
  • Biology, Medicine
  • The Biochemical journal
  • BopE is a type III secreted protein from Burkholderia pseudomallei, the aetiological agent of melioidosis, a severe emerging infection. BopE is a GEF (guanine-nucleotide-exchange factor) for the Rho GTPases Cdc42 (cell division cycle 42) and Rac1. We have determined the structure of BopE catalytic domain (amino acids 78-261) by NMR spectroscopy and it shows that BopE(78-261) comprises two three-helix bundles (alpha1alpha4alpha5 and alpha2alpha3alpha6). This fold is similar to that adopted by… CONTINUE READING
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