The growth and differentiation of cultured newborn rat keratinocytes.

  title={The growth and differentiation of cultured newborn rat keratinocytes.},
  author={Howard P. Baden and Joseph Kubilus},
  journal={The Journal of investigative dermatology},
  volume={80 2},
Keratinocytes were cultured from adult and newborn rat epidermis using the 3T3 feeder cell technique. By modifying culture conditions a long-lived line of newborn rat keratinocytes was developed which showed a plating efficiency of 40% and a doubling time of 16 h. The cells produced stratified colonies with tonofilaments, desmosomes, cell envelopes, and keratohyaline granules. When the cells were grown on a collagen gel they formed a thick stratum corneum and many keratohyaline granules. The… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 26 extracted citations


Publications referenced by this paper.
Showing 1-10 of 28 references

Fine structW"e of subcul tiva ted stratified squamous epithelium

  • A Jepson, JH MacCallum DK. Lillie
  • Exp Cell Res
  • 1980
2 Excerpts

SI: The epidermal cell which selectively adheres to a collagen substrate is the basal cell

  • JR Stanley, J-M Foidart, J CMurray, GM Martin, Katz
  • J Inve t Dermatol
  • 1980
1 Excerpt

Siaga TJ: The maintenance of a differentiated state in cul tW"ed mouse epidermal ce lls

  • DR Miller, KM Hamby, DP Alii on, SM Fischer
  • Exp Cell Res
  • 1980
1 Excerpt

Similar Papers

Loading similar papers…