The growth and differentiation of cultured newborn rat keratinocytes.

@article{Baden1983TheGA,
  title={The growth and differentiation of cultured newborn rat keratinocytes.},
  author={Howard P. Baden and Joseph Kubilus},
  journal={The Journal of investigative dermatology},
  year={1983},
  volume={80 2},
  pages={124-30}
}
Keratinocytes were cultured from adult and newborn rat epidermis using the 3T3 feeder cell technique. By modifying culture conditions a long-lived line of newborn rat keratinocytes was developed which showed a plating efficiency of 40% and a doubling time of 16 h. The cells produced stratified colonies with tonofilaments, desmosomes, cell envelopes, and keratohyaline granules. When the cells were grown on a collagen gel they formed a thick stratum corneum and many keratohyaline granules. The… CONTINUE READING

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