The green fluorescent protein.
@article{Tsien1998TheGF, title={The green fluorescent protein.}, author={Roger Tsien}, journal={Annual review of biochemistry}, year={1998}, volume={67}, pages={ 509-44 } }
In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. High-resolution crystal structures of GFP offer unprecedented opportunities to understand and manipulate the relation between…
4,649 Citations
Studying the Green Fluorescent Protein with Single-Molecule Spectroscopy
- Biology
- 2001
GFP is the only genetically encodeable protein that fluoresces without the addition of external cofactors and can be expressed in many eukaryotic and prokaryotic cells, making it an ideal marker for highly selective fluorescence labeling of live cells.
Green Fluorescent Protein-based Probes.
- Biology
- 2000
The green fluorescent protein (GFP) of the jelly fish Aequorea victoria is a spontaneously fluorescent protein that can be incorporated into other proteins by genetic fusion. One of our approaches is…
Mechanisms of protein fluorophore formation and engineering.
- Biology, ChemistryCurrent opinion in chemical biology
- 2003
Photoactivation in green to red converting EosFP and other fluorescent proteins from the GFP family
- Biology, ChemistrySPIE BiOS
- 2006
This review aims to give an overview of photoactivatable marker proteins, focusing on the molecular basis of light-induced green to red photoconversion in EosFP.
Green Fluorescent Protein and its Uses
- Chemistry, Biology
- 2013
Recognized increased opportunities to understand the relation between GFP structure and its spectroscopic function become available because of the higher resolution of its structure.
Photophysics and high-resolution spectroscopy of green fluorescent protein
- Physics
- 1999
The green fluorescent protein (GFP) has recently attracted great attention because it is the only cloned protein that exhibits strong fluorescence without any extraneous chromophores [1]. When fused…
Green fluorescent protein chimeras to probe protein-protein interactions.
- BiologyMethods in enzymology
- 2000
The Fluorescent Protein Color Palette
- BiologyCurrent protocols in cell biology
- 2007
Expansion of the fluorescent protein family to include optical highlighters and FRET biosensors further arms this ubiquitous class of fluorophores with biological probes capable of photoactivation, photoconversion, and detection of molecular interactions beyond the resolution limits of optical microscopy.
References
SHOWING 1-10 OF 203 REFERENCES
Understanding, improving and using green fluorescent proteins.
- BiologyTrends in biochemical sciences
- 1995
Fluorescent proteins from nonbioluminescent Anthozoa species
- BiologyNature Biotechnology
- 1999
We have cloned six fluorescent proteins homologous to the green fluorescent protein (GFP) from Aequorea victoria. Two of these have spectral characteristics dramatically different from GFP, emitting…
Crystal Structure of the Aequorea victoria Green Fluorescent Protein
- Chemistry, BiologyScience
- 1996
The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequorea victoria has generated intense interest as a marker for gene expression and localization of gene products. The…
GREEN FLUORESCENT PROTEIN
- BiologyPhotochemistry and photobiology
- 1995
This demonstration indicated that GFP could be used as a marker of gene expression and protein localization in living and fixed tissues and variations with more intense fluorescence or alterations in the excitation and emission spectra have been produced.
Structure and dynamics of green fluorescent protein.
- Biology, ChemistryCurrent opinion in structural biology
- 1997
Thermosensitivity of green fluorescent protein fluorescence utilized to reveal novel nuclear-like compartments in a mutant nucleoporin NSP1.
- BiologyJournal of biochemistry
- 1995
Proteins synthesized at a low temperature retained their fluorescence despite a shift to a higher temperature, and when a temperature-sensitive nsp1 mutant expressing GFP-nucleoplasmin was cultured at 23 degrees C and then shifted to 35 degrees C, this protein accumulated in novel nuclear-like compartments devoid of DNA.
Red-Shifted Excitation Mutants of the Green Fluorescent Protein
- Biology, ChemistryBio/Technology
- 1995
Using optimized combinatorial mutagenisis techniques and digital imaging Spectroscopy (DIS), we have insulated mutants of the cloned Aequorea victoria green fluorescent protein (GFP)that show…
Wavelength mutations and posttranslational autoxidation of green fluorescent protein.
- Biology, ChemistryProceedings of the National Academy of Sciences of the United States of America
- 1994
The availability of two visibly distinct colors should significantly extend the usefulness of GFP in molecular and cell biology by enabling in vivo visualization of differential gene expression and protein localization and measurement of protein association by fluorescence resonance energy transfer.
Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein.
- BiologyNucleic acids research
- 1996
A mutant of GFP with a significantly larger extinction coefficient for excitation at 488 nm with a re-engineered GFP gene sequence containing codons preferentially found in highly expressed human proteins yield an enhanced GFP which provides greater sensitivity in most systems.