The glycyl radical enzyme TdcE can replace pyruvate formate-lyase in glucose fermentation.

@article{Sawers1998TheGR,
  title={The glycyl radical enzyme TdcE can replace pyruvate formate-lyase in glucose fermentation.},
  author={Gary Sawers and C Hesslinger and Nik Muller and Myriam Kaiser},
  journal={Journal of bacteriology},
  year={1998},
  volume={180 14},
  pages={3509-16}
}
Mutants of Escherichia coli unable to synthesize a functional pyruvate formate-lyase (PFL) are severely impaired in their capacity to grow by glucose fermentation. In a functional complementation assay designed to isolate the pfl gene from Clostridium butyricum, we fortuitously identified a gene that did not encode a PFL but nonetheless was able to complement the phenotypic defects caused by an E. coli pfl mutation. The clostridial gene encoded a basic 14. 5-kDa protein (TcbC) which, based on… CONTINUE READING

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