The glycolytic flux in Escherichia coli is controlled by the demand for ATP.

  title={The glycolytic flux in Escherichia coli is controlled by the demand for ATP.},
  author={Brian J. Koebmann and Hans V. Westerhoff and Jacky L. Snoep and Dan E Nilsson and Peter Ruhdal Jensen},
  journal={Journal of bacteriology},
  volume={184 14},
The nature of the control of glycolytic flux is one of the central, as-yet-uncharacterized issues in cellular metabolism. We developed a molecular genetic tool that specifically induces ATP hydrolysis in living cells without interfering with other aspects of metabolism. Genes encoding the F(1) part of the membrane-bound (F(1)F(0)) H(+)-ATP synthase were expressed in steadily growing Escherichia coli cells, which lowered the intracellular [ATP]/[ADP] ratio. This resulted in a strong stimulation… CONTINUE READING
Highly Influential
This paper has highly influenced 10 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS


Publications citing this paper.
Showing 1-10 of 119 extracted citations


Publications referenced by this paper.
Showing 1-10 of 29 references

Experiments in molecular genetics, p. 352–355

  • J. H. Miller
  • 1972
Highly Influential
4 Excerpts

Molecular cloning: a laboratory manual, 2nd ed

  • J. Sambrook, E. F. Fritsch, T. Maniatis
  • 1989
Highly Influential
3 Excerpts

Similar Papers

Loading similar papers…