The glycolytic flux in Escherichia coli is controlled by the demand for ATP.

@article{Koebmann2002TheGF,
  title={The glycolytic flux in Escherichia coli is controlled by the demand for ATP.},
  author={Brian J. Koebmann and Hans V. Westerhoff and Jacky L. Snoep and Dan E Nilsson and Peter Ruhdal Jensen},
  journal={Journal of bacteriology},
  year={2002},
  volume={184 14},
  pages={3909-16}
}
The nature of the control of glycolytic flux is one of the central, as-yet-uncharacterized issues in cellular metabolism. We developed a molecular genetic tool that specifically induces ATP hydrolysis in living cells without interfering with other aspects of metabolism. Genes encoding the F(1) part of the membrane-bound (F(1)F(0)) H(+)-ATP synthase were expressed in steadily growing Escherichia coli cells, which lowered the intracellular [ATP]/[ADP] ratio. This resulted in a strong stimulation… CONTINUE READING
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